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KMID : 0376119950220020319
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Medical Journal of the Red Cross Hospital
1995 Volume.22 No. 2 p.319 ~ p.330
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The Regulatory Effect of Tumor Necrosis Factor and Interleukin-2 on tumor Infiltrating Lymphocytes from Renal Cell Carcinoma Patients
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Abstract
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In an attempt to increase the potency of TIL(tumor infiltrating lymphocytes) in immunotherapy, the effect of TNF(tumor necrosis factor) and IL-2(interleukin-2) were investigated in vitro on proliferation and cytotoxic effect of TIL to the various
kinds
of tumor lines and fresh frozen tumor cells from renal cell carcinoma patient. The dosage of TNF was 500 unit/ml and IL-2 was 1000 Cetus unit/ml. The cytotoxicity of TIL was checked with 4 hour Chromium release cytotoxicity assay and its
proliferation
was checked by [3H] thymidine uptake assay. For the target cell of the TIL cytotoxicity was used M-14 melanoma cell line) as a lymphokine activated killer(LAK) cell sensitive cell line for investigation of LAK activity and K562 (erythroleukemic
cell
line) for investigation of natural killer(NK) cell activity. To investigate the specific cytotoxicity on renal cell carcinoma, autologous tumor cell line was used as the autologous target, and allogeneic fresh frozen renal carcinoma cells and
renal
cell
carcinoma line (444) as the allogeneic target cells.
The proliferation effect of TIL, treated with Il-2 at 20th day of culture was ll486±591 cpm an d23817±1069 cpm wit combination of I1-2 and INF. The nonspecific cytotoxicity of TIL for the 444 at 20th day of culture was 8.2 Lytic unit(LU) in
IL-2
only
and 18.5LU with combination of I1-2 and TNF. The cytotoxicity of TIL for M-14 at 20th day of culture was 8.2Lu in IL-2 only and 19.0 LU with combination of Il-2 and TNF. The cytotoxicity of TIL for the K562 at 20th day of culture was 37.3 LU in
IL-2 has
a synergistic effect on proliferation and nonspecific cytotoxicity of TIL at 20 days of culture in vitro. These results bear important practical implication for clinical trials of TIL in adoptive immunotheraphy.
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KEYWORD
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